IMMUNOREGULATORY PROPERTIES OF N-HEXANE EXTRACT OF OSMUNDASTRUM CINNAMOMEUM IN TREATMENT OF PLASMODIUM BERGHEI INFECTION IN MICE

  • Olubukola Abibat OWOLODUN
  • Esther FAWOLE
  • Olubunmi ATOLANI
  • Abass T ANIFOWOSHE
  • Olugbenga AKINOLA
Keywords: Osmundastrum cinnamomeum, Plasmodium berghei, Immunomodulation, Inflammatory cytokines

Abstract

Malaria control is threatened by the emergence of drug-resistant parasites and there is
an urgent need for the development of antimalarial agents with novel mechanisms of
actions. This study evaluated the anti-plasmodial and immune-modulatory activities of N-
hexane leaf extract of Osmundastrum cinnamomeum in a mice model. Chloroquine-
resistant Plasmodium berghei infected mice were separated into six treatment groups
and treated orally with 0, 50, 100, 200 and 400 mg/kg of extract, water and combination
of dihydroartemisinin/ piperaquine (DHAP), respectively. Parasitological activities and
survival rates were monitored for 30 days’ post infection. Phytochemical composition of
O. cinnamomeum was analysed by gas chromatography-mass spectrometry (GC-MS)
methods. Levels of TNF-α and IL-10 were assessed using enzyme-linked immunosorbent
assay (ELISA). Leaf extract of O. cinnamomeum is rich in terpenoids, saponins and
cardiac glycosides. The extract showed significant (p<0.05) antiplasmodial effect in the
treated groups relative to parasitemia (23.68 %) in the untreated control on day 13.
Parasitaemia was significantly higher in the DHAP group (9.83 %) on day 30 compared to
extract treatment of 50 mg/kg (2.09 %) and 100 mg/kg (1.83 %). Significantly low level
of TNF-α (28.82 pg/ml) and conversely, high expression of IL-10 (79.04 pg/ml) were
recorded in the 50 mg/kg test group. There was a significantly higher survival rate of
animals in the same group (50 mg/kg). In conclusion O. cinnamomeum demonstrated
potential activity to suppress parasite and also prime the immune system against malaria
infection in mice. Therefore, O. cinnamomeum may be used as a potential adjunctive
therapy in the treatment of malaria infection .

Published
2019-01-14