ANTIDIABETIC EFFECT OF Sarcocephalus latifolus AQUEOUS ROOT EXTRACT IN EXPERIMENTAL RAT MODEL

Adaku Vivian IWUEKE, Fred Okwesili NWODO

Abstract


Investigations were carried out to evaluate the blood sugar lowering activity of the aqueous extract of Sarcocephalus latifolus roots (SLA) in normal and streptozotocin-diabetic rats. The extract (250 mg/kg body weight; p.o) caused 76 % reduction in blood glucose within 6h in fasted diabetic rats. However, the extract at the same dose showed no effect in normorglycaemic rats. Acute toxicity studies of the extract in mice gave an oral LD50 value greater than 5000 mg/kg. Phytochemical tests revealed that SLA tested positive for alkaloids, tannins, saponins, terpenoids, reducing sugars, carbohydrates, sterols and glycosides. The results show that the aqueous root extract of S latifolus has blood glucose lowering effect, which is consistent with the use of the root in folklore diabetes management.


Full Text:

PDF

References


ABREU, P. and PEREIRA, A. (2001). New Indole Alkaloids from Sarcocephalus latifolius. Natural Products Letters, 15(1): 43 – 48.

AKABUE P. and MITTAL G. C. (1982). Clinical Evaluation of a Traditional Herbal Practice in Nigeria: A preliminary Rep. Journal of Ethnmopharmacology, 6(3): 355 – 359.

ASUBIOJO, O. J., GUINN, V. P. and OKUNUGA, A. (1982). Multi-element analysis of Nigerian chewing sticks by instrumental neuron activation analysis. Journal of Radiology and Analytical Chemistry, 74: 149 – 156.

BALKAU B., CHARLES M. A. and ESCHIWEGA, E. (2000). Discussion epidemiologique des nouveaux criteres du diabete. Mt Endocrinologie, 2: 229 - 234.

BOYE, G. L. (1990). Studies on antimalarial action of Cryptolepis sanguinolenta extract. Pages 243 – 251. In: Proceedings of the International Symposium on East-West Medicine. Seoul, Korea.

EVANS, W. C. (1989). Trease and Evans Pharmacognosy. 13th Edition. Bailliere Tindall, London.

GIDADO, A., AMEH, D. A. and ALAWODI, S. E. (2005). Effect of Nauclea latifolia leaves aqueous extracts on blood glucose levels of normal and alloxan induced diabetic rats. African Journal of Biotechnology, 4 (1):

GUPTA, M. P., SOLIS, N. G., ESPOSITO, M. and SANCHEZ, S. (1984). Hypoglycaemic activity of Neurolaena lobata (L) R. British Journal of Ethnopharmacology, 10: 323 – 327.

HARBORNE, J. B. (1998). Phytochernical methods. A guide to modern techniques of plant analysis. Chapman and Hall Limited, London.

HOTELLIER, F., DELAVEAU, P. and POUSSET J. L. (1979). Alkaloids and glycoalkaloids from leaves of Nauclea latifolia. Planta Medica, 35: 242 – 250.

KAMESWARA R., KESAVULU, M. M., GIRI, R. and RAO, C. (1999). Antidiabetic and hypolipidemic effects of Momordica cymbalaria Hook, fruit powder in alloxan diabetic rats. Journal of Ethnopharmacology, 67: 103 – 109.

KOKWARO J. O. (1976). Medicinal Plants of East Africa. East African Literature Bureau, Nairobi.

KRISHNA B., NAMMI, S., KOTA, M. K. and KRISHNA RAO R. V. (2004). Evaluation of hypoglycaemic and antihyperglycaemic effects of Datura metel Linn seeds in normal and alloxam-induced diabetic rats. Journal of Ethnopharmacology, 9: 95 – 98.

LORKE, D. (1983). A New approach to acute toxicity testing. Archives of Toxicology, 54: 275 – 287.

MADUBUNYI I. I. (1995). Anti-hepatotoxic and trypanocidal activities of the ethanolic extract of Nauclea latifolia root bark. Journal of Herbs Spices and Medicinal Plants, 3(2): 23 – 53.

MORAH F. N. (1995). Naucledal and epinaucledal from an antiviral preparation from Nauclea latifolia. Global Journal of Pure and Applied Science, 1(1 – 2): 59 – 62.

OKYAR, A., CAN, A., AKER. N., BAKTIR, G. and SUTLUPINAR, N. (2001). Effect of Aloe vera leaves on blood glucose levels in type I and type II diabetic rat models. Phytotherapy Research, 15: 157 – 161.

PEPATO M. T., BAVIERA A. M., VENDRAMINI R. C., PEREZ, M. P., KETTELHUT, I. C. and BRUNETTI, I. J. (2003). Cissus sicyoides (Princess wine) in long term treatment of streptozotocin-diabetic rats. Biotechnology and Applied Biochemistry, 37: 15 – 20.

PRINCE, P. S. M., MENON, V. P. and GUNASEKHARAN, G. (1999). Alloxan diabetic rats. Journal of Ethnopharmacology, 64: 53 – 57.

RANG, H. P. and DALE, M. M. (1991). The Endocrine System Pharmacology, 2nd Edition, Longman, Harlow.

RAO, B. K. and RAO, A. (2001). Hypoglycemic and antihyperglycaemic activity of Syzygium alternifolium (Wt) walp seed extracts in normal and diabetic rats. Phytomedicine, 8(2): 88 – 93.

SCOPPOLA, A., MONTECCHI, F. R., MEZINGER, G. and LALA, A. (2001). Urinary mevalonate excretion rate in type II diabetes; role of metabolic control; Atherosclerosis, 156: 357 – 461.

SUBRAMANIAN, A., PUSHPAGANDAN, P., RAGASEKHARAN, S., EVANS D. A., LATHA P. G. and VALSARAJ, R. (1996). Effects of Artemisia pallens wall on blood glucose levels in normal and alloxan-induced diabetic rats. Journal of Ethnopharmacology, 50:13 -17.


Refbacks

  • There are currently no refbacks.


A Publication of Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State, Nigeria.