A NEW AND SIMPLE METHOD OF CONFIRMATORY DETECTION OF MATING IN ALBINO RATS (Rattus norvegicus)

Izuchukwu Shedrack OCHIOGU, Chukwuka Nwocha UCHENDU, John Ikechukwu IHEDIOHA

Abstract


A new and simple method of detecting mated female albino rats was developed and tested for precision and accuracy. The method involved the gross observation of grey to yellowish protein coagulates (remnants of the copulatory plug) on vaginal smears of mated females made on clean glass slides. Results of test of the new method showed that the mean length of time between observation of the protein coagulates on vaginal smears and delivery of the mated pregnant females was 21.39  ± 0.11 days (range = 20 – 23 days, n = 58), which concurred with the normal 21-day gestation length of rats. The coefficient of variation (CV) of imprecision of the new method was found to be 3.74 %. This new method is simple, easy to apply and does not interfere with fertilization and pregnancy, and also does not involve either the use of specially designed rat cages or microscopy of vaginal smears, which were the constraints of the former methods of confirming mating in rats.

 


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References


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A Publication of Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State, Nigeria.