ACETYLSALICYLIC ACID AND CELLULAR DAMAGE IN KIDNEY OF METABISULPHITE TREATED RATS

Joseph Eniola OLAJIDE, Adewumi Musbau AKANJI, Momoh SANNI, James OMALE

Abstract


The effect of acetylsalicylic acid (ASA) as membrane stabilizers was investigated on the kidney of experimental rats treated with sodium metabisulphite. Administration of sodium metabisulphite has been shown to labilize the plasma membrane of some rat tissues. Sodium metabisulphite (10 mg/kg b.wt) acetylsalicylic while both chemical substances of same dose were both chemicals were concurrently administered to three group of rats for two weeks (14 days) while the fourth (4th) group of rats served as control and were given physiological saline alone. Two ‘marker’ enzymes, alkaline phosphatase (ALP) and acid phosphatase (ACP) activities were spectrophotometrically determined to monitor the efficacy of acetylsalicylic acid in membrane stabilization. Following the initial administration of metabisulphite alone, immediate significant decreases (p < 0.05) in ALP activities were observed. The activity latter recovered towards control value by the tenth day. For ACP, the loss in activities was sustained throughout the experimental period. However, the difference showed no significant difference (p > 0.05). In acetylsalicylic acid administered rats the activities of ALP were higher than for the control group while the activities of ACP were not appreciably affected. The combined treatment gave values that were not significantly different from the control values (p < 0.05).


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ABOU-ELENIN, K., XYDAKIS, A., HAMDY, O. and HORTON, S. (2002). The effect of aspirin and various iontophoresis solution vehicles on skin microvascular reactivity. Microvascular Research, 63: 91 – 95.

AKANJI, M. A. (1986). A comparative biochemical study of the interaction of some trypanocides with rats tissues cellular systems. PhD Thesis. University of Ile-Ife Nigeria, Total Pages 186.

AKANJI, M. A. and NLUMANZE, S. E. (1987). Alkaline phosphatase activities repeated Suramin administration in some rat tissues cellular systems. Pharmacology and Toxicology, 61: 182 – 183.

AKANJI, M. A. and NGAHA, E. O. (1989). Effect of repeated administration of Berenil on urinary excretion with corresponding tissue pattern in rats. Pharmacology and Toxicology, 64: 272 – 275.

AKOGYERAM, C. and SOUTHERLAND, W. M. (1980). The interaction of bisulphate with membrane lipids. Abstract of Federated Proceedings of American Society of Experimental Biology, 39: pp 1836.

BHAGAT, B. and LOCKETT, M. F. (1964). The effect of sulphite in solid diets on the growth of rats. Food Cosmetic and Toxicology, 2: 1 – 13.

BAGHAT, K., COLLIER, J. and VALLANCE, P. (1995). Vasodilatation to arachidonic acid in humans: An insight into endogenous prostanoids and effects of aspirin. Circulation, 92: 2113 – 2118.

CLEGG, A. G. and CLEGG, P. C. (1975). Biology of the mammal. 4th edition England Book Society and Heinemann Medical Books Limited. Great Britain. pp 387 – 395

De DUVE, C. B. C., WATTIUX, R. and BAUDLIN, P. (1962). Distribution of enzyme between sub cellular fractions in animal tissues. Advanced Enzymology, 24: 241 – 358.

DURAND, S., FROMY, B., KOITAL, A., ABRAHAM, P. and SAUMET, J. L. (2002a). Oral single high – dose aspirin results in a long – lived inhibition of anodal current – induced vasodilatation. British Journal of Pharmacology, 137: 384 – 390.

DURAND, S., FROMY, B., KOITAL, A., ABRAHAM, P. and SAUMET, J. L. (2002b). Vasodilatation in response to repeated anodal current application in the human skin relies on aspirin – sensitive mechanisms. Journal of Physiology, 540: 261 – 269.

FOLLEY, S. J. and KAY, H. D. (1935). The alkaline phosphomonoesterase of Mammary gland. Biochemical Journal, 29: 1837 – 1850.

GEORGE, J. B. (2002). Basic food Microbiology, 2nd edition, Chapman and Hall Incorporated, New York.

GROMAL, A. G., BARDWILL, C. J. and DAVID, M. M. (1949). Determination of Serum protein by means of the buiret reaction. Journal of Biological Chemistry, 177: 571 – 766

GUNNISON, A. F. (1981). Sulphites toxicity: A critical review invitro and in vivo data. Food Cosmetic and Toxicology, 19: 221 – 232

HALLWELL, B. (1974). Super – oxide dismutase, catalase and glutathione peroxidase – solutions to problem of living with oxygen. New Phytology, 73: 1075 – 1086.

HALLWELL, B. (1978). Biochemical mechanisms accounting for toxic action of oxygen on living organisms: the key role of super –oxide dismutase. Cellular Biology International Report, 2: 115 – 127.

HARKNESS, D. R. and ROTH, S. (1969). Purification and properties of 2,3-diphosphoglyceric acid phosphatase from human erythrocytes. Biochemistry and Biophysics Research Communications 34: 849 – 856

HLA, T. T. and BAILEY, J. M. (1989). Differential recovery of prostacyanin synthesis in cultural vascular endothelial versus smooth muscle cells after inactivation of cyclooxygenase with aspirin. Essential Fatty Acids, 36: 175 – 184.

IGNARRO, L. S. (1971): Effect of anti-inflammatory drugs on the stability of rat liver lysosomes in vitro. Biochemistry and Pharmacology, 20: 2847 – 2853.

KAPLAN, M. M. (1972). Alkaline phosphatase. New England Journal of Medicine, 280: 200 – 2002.

KAPLAN, D., MEJILTON, C. and LUTCHTEL, D. (1975). Bisulphite induced lipid oxidation. Archives of Environmental Health, 30: 507 – 509.

KHAN, A. U. (1970). Singlet molecular oxygen from superoxide and sensitized fluorescence of organic molecules. Science, 168: 476 – 477.

MILLER, W. S. and SMITH, J. G. (1966). Effects of acetylsalicylic acid on lysosomes. Proceedings of Society of Experimental Medicine, 122: 634 – 640

NGAHA, E. O (1979). Toxic renal damage. Nigerian Medical Journal, 9: 407 – 414.

NGAHA, E. O. (1982). Some biochemical changes on the rat during repeated chloroquine administration. Toxicology Letters, 10: 145 – 149.

NGAHA, E. O., FRY, M. and PLUMMER, D. T. (1979). The effect of cephaloridine on the stability of rat kidney lysosomes. Chemistry and Biology Interactions, 24: 199 – 208.

OLAGOKE, O. B. (1991). The mode of cellular toxicity of sodium metabisulphite on some rat tissues. M.Sc Thesis, University of Ilorin, Ilorin, Nigeria. 87 pp.

PATRONO, C., COLLAR, B., DALEN, J. E. and ROTH, G. (1998). Platelet active drugs: The relationship among dose, effectiveness and side effects. Chest, 114: 470s – 488s.

PFLEIDERER, G., WIELAND, T. and JECKEL, D. (1956). Toxicity of sulphites. Biochemistry, 7: 287 - 298

ROGER, H. J., SPECTRE, R. G. and TROUNCE, J. R. (1981). Salicylates: A textbook of clinical Pharmacology. Academy Press, New York. pp. 281 – 286.

SCHANKER, L. S., SHORE, P. A. and BRODIE, B. B. (1957). Absorption of drugs from the stomach of rat. Journal of Pharmacology and Experimental Therapy, 120: 528 – 532

SHIBKO, S. and TAPPEL, A. L. (1965). Rat Kidney lysosomes: Isolation and Properties. Biochemistry Journal, 95: 731 – 741

TAYLOR, S. J., HIGLEY, N. A. and BUSH, R. K. (1986). Sulphites in food: Uses, analytical methods, residue, fate, exposure assessment, metabolism, toxicity and hypersensitivity. Advance Food Research, 30: 1 – 8

WEDRIHA, B. L. (1984). Chemistry of sulphur dioxide on foods. Elsevier Applied Science Publishers, Barking Essex. pp 214 – 215.

WILLIAM, C. F. and DENNIS, C. W. (1995). Food Microbiology, 4th Edition; Tata and McGraw-Hill Publishing Company Limited, New Delhi. pp 150 – 152.

WILKINSON, J. H. (1963). An introduction to diagnostic enzymology. Arnold E. Limited, London. pp 96 – 105.

WRIGHT, P. J., LEATHWOOD, F. D. and PLUMMER, D. T. (1974). Enzymes in rat urine: acid phosphatase. Enzymologia, 42: 317 – 327

WRIGHT, P. J. and PLUMMER, D. T. (1974). The use of urinary enzyme measurement to detect renal damage by nephrotoxic compounds. Biochemistry and Pharmacology, 23: 65 – 73.

WRIGHT, R., ALBERT, K. G. M., MARRAN, S. and MILL-WARDSANDLER, G. H. (1979). Liver and biliary disease. W.B. Sanders Company Limited, London.


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A Publication of Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State, Nigeria.